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Support
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Single Cell Gene Expression
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Index
Q&A
CONTACT SUPPORT
Index
10x Genomics Sample Preparation Products
Chromium Nuclei Isolation Kit How-to Videos
Chromium Nuclei Isolation Kit Sample Prep User Guide
Chromium Nuclei Isolation Kit Tested Tissues
Technical Notes
Assay Scheme and Configuration of Chromium Single Cell 3' v2 Libraries
Base Composition of Sequencing Reads of Chromium Single Cell 3’ v2 Libraries
Best Practices to Minimize Chromium Next GEM Chip Clogs and Wetting Failures
Biological & Technical Variation in Single Cell Gene Expression Experiments
Chromium Connect: Consistent Automated Single Cell Gene Expression Library Generation
Chromium Connect: Instructions for Bypassing Consumable Scanning
Chromium Connect: Modular Gene Expression Workflow & Data Overview
Chromium Connect: Single Cell 3' Gene Expression Dual Index Library Data Overview
Chromium Next GEM Single Cell 3' HT v3.1: Reagents, Workflow & Data Overview
Chromium Next GEM Single Cell 3' LT v3.1: Reagents, Workflow, & Data Overview
Chromium Next GEM Single Cell 3ʹ v3.1: Cell Multiplexing
Chromium Next GEM Single Cell 3ʹ v3.1: Dual Index Libraries
Chromium Next GEM Single Cell 3ʹ v3.1: Reagent & Workflow Updates
Chromium Single Cell 3ʹ v3 Reagent, Workflow & Software Updates
Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® NovaSeq®
Chromium Single Cell 3’ v2 Libraries – Sequencing Metrics for Illumina® Sequencers
Chromium Single Cell 3’ v2 Libraries – Sequencing Performance on Illumina® NextSeq® 500 Flow Cells
Chromium Single Cell 3’ v2 Libraries – Sequencing Performance on Illumina® NextSeq® 500 Flow Cells
Chromium Single Cell 3’ v3 Libraries – Sequencing Metrics for Illumina® Sequencers (pending)
Chromium Single Cell Applications - Guidelines for Optimal Sample Preparation
Chromium Single Cell CRISPR Screening – Experimental Planning Guide
Chromium X Series: Network Connectivity Guidelines
Chromium X Series: Single Cell Data Highlights and Comparisons
Chromium™ Single Cell Applications
Evaluating Short-term Storage of Cells for Single Cell Sequencing
Guide RNA Specifications Compatible with Feature Barcoding technology for CRISPR Screening
Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions
Importance of Cell Stock Concentration for Accurate Target Cell Recovery
Instructions to Download and Process BAM files of 1.3 Million Brain Cells
Instructions to Download and Process FASTQs of 1.3M Brain Cells
Instructions to Download and Process FASTQs of Chromium X Demonstration Data
Interpreting Cell Ranger Multi Web Summary Files for Single Cell 3’ Gene Expression with Feature Barcode technology for Cell Multiplexing
Interpreting Cell Ranger Web Summary Files for Single Cell Gene Expression Assays
Interpreting Intronic and Antisense Reads in 10x Genomics Single Cell Gene Expression Data
Interpreting Single Cell Gene Expression Data With and Without Intronic Reads
Neutrophil Analysis in 10x Genomics Single Cell Gene Expression Assays
Performance of Blood Collection Tubes and PBMC Isolation Methods for Single Cell Gene Expression
Pipette Tip Recommendations for 10x Genomics Single Cell Protocols
Quality Control of Cell Surface Protein Labeling using Flow Cytometry
Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications
Resolving Cell Types as a Function of Read Depth and Cell Number
Sequencing Metrics & Base Composition of Single Cell 3' v3.1 Dual Index Libraries
Thermal Cycler Recommendations for 10x Genomics Single Cell and Spatial Protocols
User Guides
Automated Gene Expression Library Construction
Chromium Connect Document Resources
Chromium Connect Quick Reference Cards
Chromium Connect User Guide
Chromium Controller Readiness Test
Chromium Controller Readiness Test with Chromium Next GEM Test Chip
Chromium Controller Training Kit
Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)
Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry) - Draft for new Support site
Chromium Next GEM Automated Single Cell 3ʹ cDNA Kit v3.1 User Guide Supplement
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index)
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening & Cell Multiplexing
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein
Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein & Cell Multiplexing
Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide
Chromium Next GEM Single Cell 3ʹ LT Reagent Kits (v3.1 Dual Index) with Feature Barcode technology for Cell Surface Protein
Chromium Next GEM Single Cell HT Training Reagent Kits User Guide
Chromium Next GEM Training Kit User Guide
Chromium Single Cell 3' Reagent Kits User Guide (v2 Chemistry)
Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry)
Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for CRISPR Screening
Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index)
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for CRISPR Screening and Cell Multiplexing
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Multiplexing
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Cell Multiplexing
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry)
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening
Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3' v2 Reagent Kit, Quick Reference Cards (v2 Chemistry)
Chromium Single Cell Controller Readiness Test
Chromium X Series (X/iX) Quick Reference Cards
Chromium X Series (X/iX) User Guide with Readiness Test
Chromium™ Single Cell 3' Protocol Time Planner (v1 Chemistry)
Chromium™ Single Cell 3' Reagent Kit (v1 Chemistry)
Chromium™ Single Cell 3' Reagent Kit, Quick Reference Cards (v1 Chemistry)
Flowcell Capacity Calculator
Demonstrated Protocols
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing
Moss Protoplast Suspension for Single Cell RNA Sequencing
Nuclei Isolation from Adult Mouse Brain Tissue for Single Cell RNA Sequencing
Plate-based Sample Preparation for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
Single Cell Gene Expression Demonstrated Protocol Compatibility Table
Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table
Single Cell Gene Expression LT Demonstrated Protocol Compatibility Table
Single Cell Protocols - Cell Preparation Guide
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
Tumor Dissociation for Single Cell RNA Sequencing
Specifications
Chromium Connect Declaration of Conformity
Chromium Connect Specifications
Chromium Controller Declaration of Conformity
Chromium Controller Specifications
Chromium Single Cell Controller Specifications
Chromium X Series (X/iX) Declaration of Conformity
Chromium X Series (X/iX) Specifications
Chromium X Series (X/iX) UKCA Declaration of Conformity
Sample Index Sets for Single Cell 3'
Sequencing Requirements for Single Cell 3'
Sequencing Requirements for Single Cell 3' HT
Sequencing Requirements for Single Cell 3' LT
Instrument Softwares
Chromium Connect Instrument Software Update Procedure
Chromium Connect Software Release Note
Product Notifications
Chromium Connect Product Notification
Chromium Connect Product Notification: User Interface Screens
Training Module Chromium Next GEM Single Cell 5' v2 Dual Index
Training Module Single Cell Gene Expression
Workflow Update for Chromium Next GEM Chip and Gasket Assembly
Getting Started
Chromium Connect Quick Reference Cards
Getting Started: Single Cell 3’ Gene Expression
Next GEM Technology for Single Cell Gene Expression
On-Demand Training for Single Cell Gene Expression
Single Cell Gene Expression HT with Feature Barcode technology
Single Cell Gene Expression HT with Feature Barcode technology
Single Cell Gene Expression LT with Feature Barcoding technology
Single Cell Gene Expression LT with Feature Barcoding technology
Single Cell Gene Expression with Cell Multiplexing technology
Single Cell Gene Expression with Feature Barcoding technology
Targeted Gene Expression Overview for Single Cell Gene Expression
Firmware Upgrades
Chromium Controller Firmware Release Note
Chromium Controller Firmware Update Procedure
Chromium X Series Firmware Release Note
Chromium X Series Firmware Update Procedure
SDS
Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits Safety Data Sheets (v3.1 Chemistry)
Chromium Next GEM Single Cell 3' HT Reagent Kits Safety Data Sheets (HT v3.1 Dual Index Chemistry)
Chromium Next GEM Single Cell 3' LT Reagent Kits Safety Data Sheets (LT v3.1 Dual Index Chemistry)
Chromium Next GEM Single Cell 3' Reagent Kits Safety Data Sheets (v3.1 Chemistry)
Chromium Next GEM Single Cell 3' Reagent Kits Safety Data Sheets (v3.1 Dual Index Chemistry)
Chromium Next GEM Single Cell HT Training Reagent Kits Safety Data Sheets
Chromium Next GEM Training Kit Safety Data Sheets
Chromium Nuclei Isolation Kit Safety Data Sheets
Chromium Training Kit Safety Data Sheets
Other
Guidance for Instruments with Software v20211214 and v20220114
Software
Overview
System Requirements
Downloads
Datasets
Datasets