Evaluating Short-term Storage of Cells for Single Cell Sequencing

Technical Note, Last Modified on January 4, 2022, Permalink

CG000481_TechNote Short Term Cell Storage_RevA.pdf

Cryopreservation is a common method for preserving samples for long-term storage and transport. However, some cell types may be sensitive to freeze-thaw cycles or may need to be stored for only a few hours, highlighting a need for alternative methods of sample preservation. Protocols for shortterm storage of cells without cryopreservation are limited, and the impact of these on single cell sequencing data has not been thoroughly evaluated. This Technical Note evaluates HypoThermosol FRS and RPMI + 10% FBS as short-term cell storage solutions in 10x Genomics Single Cell 3' Gene Expression Assays.