Quality Control of Cell Surface Protein Labeling using Flow Cytometry

Technical Note, Feature Barcoding technology, Last Modified on May 11, 2022, Permalink

CG000231_TechnicalNote_QualityControlCellSurfaceProteinLabeling_RevA.pdf

Cell surface proteins can be labeled with a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide, to generate Chromium Single Cell 3' or 5' Cell Surface Protein libraries. This Technical Note outlines methods to perform quality control of cell surface protein labeling with antibody-oligonucleotide conjugates using flow cytometry, along with guidelines for determining optimal labeling concentration.

FOR USE WITH:

• Chromium Single Cell 3' Reagent Kits v3 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000185)

• Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000206)

• Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide with Feature Barcoding technology for Cell Surface Protein (CG000317)

• Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000417)

• Chromium Single Cell V(D)J Reagent Kits User Guide with Feature Barcoding technology for Cell Surface Protein (CG000186)

• Chromium Next GEM Single Cell V(D)J Reagent Kits User Guide v1.1 with Feature Barcoding technology for Cell Surface Protein (CG000208)

• Chromium Next GEM Single Cell V(D)J Reagent Kits User Guide v2 (Dual Index) with Feature Barcoding technology for Cell Surface Protein (CG000330)

• Chromium Next GEM Single Cell 5' HT Reagent Kits v2 with Feature Barcode technology for Cell Surface Protein (CG000424)