Sequencing

Specifications

• Sequencing Requirements for Single Cell V(D)J
• Sequencing Requirements for Immune Profiling HT
• Sample Index Sets for Single Cell Immune Profiling

User Guide

• Flowcell Capacity Calculator

Technical Note

• Interpreting Single Cell Gene Expression Data With and Without Intronic Reads
• Instructions to Download and Process FASTQs of Chromium X Demonstration Data

Gene Expression

• Interpreting Cell Ranger Web Summary Files for Single Cell Gene Expression Assays
• Resolving Cell Types as a Function of Read Depth and Cell Number
• Interpreting Intronic and Antisense Reads in 10x Genomics Single Cell Gene Expression Data

Sequencing Metrics

• Sequencing Metrics & Base Composition of Single Cell 5' v2 Dual Index Libraries
• Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® Sequencers
• Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® NovaSeq®
• Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries