Specifications, Last Modified on August 22, 2022, Permalink
Dual index plates
Dual index plates are used with Chromium Single Cell 5' v2 and 5' HT v2 Dual Index libraries.
Each well of a dual index plate contains a mix of one unique i7 and one unique i5 sample index.
The files below contain the mappings from the sample index names to the associated set of Index (i7) and Index 2 (i5) oligonucleotides. Both the forward and reverse complement sequences are listed for the i5 sequence. Please see this article for more information.
Please note that the sample index plate used for 5' v2 and 5' HT v2 Dual Index Gene Expression and V(D)J libraries is different from the sample index plate used for 5' v2 and 5' HT v2 Dual Index Cell Surface Protein libraries. Always make sure to select the correct sample index plate for the appropriate library type.
5' v2 and 5' HT v2 Dual Index Gene Expression, V(D)J, and CRISPR libraries are made using the following sample index plate:
Single Cell 5’ v2 and 5' HT v2 Dual Index Cell Surface Protein libraries are made using the following sample index plate:
Single index plates
Single index plates are used with Chromium Single Cell 5' v1 and v1.1 libraries.
Sample indexes for use with the Chromium Single Cell 5' v1 and v1.1 reagent kits each comprise four oligonucleotides. When analyzing sequencing data for a given sample, you must combine the reads associated with all four of the oligonucleotides in the sample index.
Please note that the sample index plate used for V(D)J Enriched and 5’ Gene Expression libraries are different from the sample index plate used for 5' Cell Surface Protein and Antigen Specificity libraries.
5' v1.1 Gene Expression and V(D)J enriched libraries are made using the following sample index plate:
5' v1.1 Cell Surface Protein and Antigen Specificity libraries are made using the following sample index plate:
5' v1 Gene Expression and V(D)J enriched libraries are made using the following sample index plate:
5' v1 Cell Surface Protein and Antigen Specificity libraries are made using the following sample index plate: