Sequencing Metrics & Base Composition of Single Cell 5' v2 Dual Index Libraries

Technical Note, Sequencing Metrics, Last Modified on June 27, 2022, Permalink

CG000401_TechNote_SeqMetrics_SingleCell5-v2.0_DualIndex_RevC.pdf

The Chromium Next GEM Single Cell 5' v2 (Dual Index) workflow produces sequencing-ready Gene Expression, V(D)J, and/or Cell Surface Protein libraries from the same single cells. This enables simultaneous profiling of cellular features in combination with gene expression profiling. This Technical Note presents a comparison of sequencing metrics for various Single Cell 5' v2 Dual Index library types across Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.

FOR USE WITH:

• Chromium Next GEM Single Cell 5' Kit v2, 16 rxns PN-1000263
• Chromium Next GEM Single Cell 5' Kit v2, 4 rxns PN-1000265
• Library Construction Kit, 16 rxns PN-1000190
• 5' Feature Barcode Kit, 16 rxns PN-1000256
• Chromium Single Cell Human TCR Amplification Kit, 16 rxns PN-1000252
• Chromium Single Cell Human BCR Amplification Kit, 16 rxns PN-1000253
• Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns PN-1000254
• Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns PN-1000255
• Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286
• Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287
• Dual Index Kit TT Set A, 96 rxns PN-1000215
• Dual Index Kit TN Set A, 96 rxns PN-1000250