Sample Prep

10x Genomics Sample Preparation Products

Chromium Nuclei Isolation Kit

• Chromium Nuclei Isolation Kit Sample Prep User Guide
• Chromium Nuclei Isolation Kit Tested Tissues
• Chromium Nuclei Isolation Kit How-to Videos

Demonstrated Protocol (19 documents)

• Single Cell Gene Expression Demonstrated Protocol Compatibility Table
• Single Cell Protocols - Cell Preparation Guide
• Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
• Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
• Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing
• Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling
• Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
• Tumor Dissociation for Single Cell RNA Sequencing
• Single Cell Gene Expression LT Demonstrated Protocol Compatibility Table
• Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table
• Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
• Plate-based Sample Preparation for Single Cell RNA Sequencing
• Nuclei Isolation from Adult Mouse Brain Tissue for Single Cell RNA Sequencing
• Moss Protoplast Suspension for Single Cell RNA Sequencing
• Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
• Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing
• Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols

Technical Note (14 documents)

• Pipette Tip Recommendations for 10x Genomics Single Cell Protocols
• Performance of Blood Collection Tubes and PBMC Isolation Methods for Single Cell Gene Expression
• Neutrophil Analysis in 10x Genomics Single Cell Gene Expression Assays
• Evaluating Short-term Storage of Cells for Single Cell Sequencing

v2 Chemistry

• Chromium™ Single Cell Applications

Feature Barcoding technology

• Quality Control of Cell Surface Protein Labeling using Flow Cytometry
• Chromium Single Cell CRISPR Screening – Experimental Planning Guide
• Guide RNA Specifications Compatible with Feature Barcoding technology for CRISPR Screening

Gene Expression

• Chromium Single Cell Applications - Guidelines for Optimal Sample Preparation
• Resolving Cell Types as a Function of Read Depth and Cell Number
• Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications
• Importance of Cell Stock Concentration for Accurate Target Cell Recovery
• Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions
• Biological & Technical Variation in Single Cell Gene Expression Experiments

SDS

• Chromium Nuclei Isolation Kit Safety Data Sheets