Single Cell Gene Expression LT Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on June 17, 2022, Permalink

Protocol	SC3'LTv3.1	SC3'LTv3.1 with Cell Surface Protein
Single Cell Protocols - Cell Preparation Guide	✓	✓
Methanol Fixation of Cells for Single Cell RNA Sequencing	-	✘
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing	✓*	-
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling	✓*	-
Tumor Dissociation for Single Cell RNA Sequencing	✓*	✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing	✓	✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing	✓	✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing	-	✘
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing	✓	-
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing	✓	✓
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing	✓	✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols	✓	✓
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing	✓	✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols	**	**

✓ Compatible (tested in-house) ✓* Untested but low risk ** Compatible but unsupported - Untested, use at your own risk ✘ Incompatible