Resolving Cell Types as a Function of Read Depth and Cell Number

Technical Note, Gene Expression, Last Modified on September 24, 2019, Permalink

CG000148_10x_Technical_Note_Resolving_Cell_Types_as_Function_of Read_Depth_Cell_Number_RevA.pdf

Technical Note - Resolving Cell Types as a Function of Read Depth and Cell Number

Chromium™ Single Cell Solutions enable gene expression profiling of 100–10,000 cells per reaction. When sequencing costs or capacity are limiting, there is often a trade-off between sequencing a larger cell number (breadth) and sequencing a smaller cell number with more reads (depth). Here, we examine how sequencing depth and cell number influence the detection of major cell types in peripheral blood mononuclear cells (PBMCs).

FOR USE WITH