Chromium Next GEM Single Cell V(D)J v1.1: Reagent & Workflow Updates

Technical Note, Last Modified on February 4, 2020, Permalink

CG000228_TechNote_ChromiumNextGEMSingle Cell V(D)J v1.1_Reagent_Workflow_Updates_RevC.pdf

The Chromium Single Cell Immune Profiling Solution provides a comprehensive, scalable solution to simultaneously examine the cellular context of the immune system and the immune repertoires of hundreds to tens of thousands of cells on a cell-by-cell basis. This document highlights the key changes in Chromium Next GEM Single Cell V(D)J v1.1 reagents, along with workflow updates for generating and analyzing Illumina-ready sequencing libraries.

FOR USE WITH:

• Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 16 rxns PN-1000165
• Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1, 4 rxns PN-1000167
• Chromium Single Cell 5’ Library Construction Kit, 16 rxns PN-1000020
• Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005
• Chromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns PN-1000016
• Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell, 96 rxns PN-1000071
• Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell, 96 rxns PN-1000072
• Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120
• Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127
• Single Index Kit T Set A, 96 rxns PN-1000213
• Chromium Single Cell 5' Feature Barcode Library Kit, 16 rxns PN-1000080
• Single Index Kit N Set A, 96 rxns PN-1000212