Chromium Connect Automated Single Cell 5' v2 with Feature Barcode technology: Workflow and Data Overview
Technical Note, Last Modified on June 24, 2022, Permalink
Chromium Next GEM Single Cell 5' v2 automated workflow with Feature Barcode technology is now available on the Chromium Connect Automated System. This automated workflow allows measuring cell surface protein expression and antigen specificity while simultaneously profiling single cell gene expression along with V(D)J immune repertoire mapping from the same pool of cells. This Technical Note shows comparable gene expression, immune repertoire, and cell surface protein expression data obtained using manual and automated Chromium Next GEM Single Cell 5' v2 with Feature Barcode technology workflow. In addition, the document shows cell surface protein expression data derived from automated single cell libraries generated from complex samples for immunophenotyping as well as for mapping antigen specific immune repertoire.