Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries

Technical Note, Sequencing Metrics, Last Modified on June 24, 2022, Permalink

CG000103_10x_Technical Note_Base Composition_VDJ.pdf

Technical Note - Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries

This Technical Note describes base percentage profiles and Phred quality scores (shown as %Q30 in Illumina SAV software) that are characteristic of Chromium Single Cell V(D)J libraries for the recommended sequencing run configuration. Chromium Single Cell V(D)J libraries incorporate standard Illumina paired-end constructs with P5 and P7 sequences at opposite ends. The 16bp 10x Barcode and the 10bp UMI are located at the start of Read 1, while sample index sequence information is incorporated into the i7 index read. Read 1 and Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing.

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