Immune Profiling HT with Feature Barcode technology
Selected Resources for HT (High Throughput)
Getting Started, Last Modified on December 13, 2021, Permalink
What is Single Cell Immune Profiling HT?
- Profile 2,000 to 20,000 single cells to detect rare clonotypes and cell type sub-populations per sample
- Simultaneously characterize gene expression and enrich for single TCR and Ig cells providing paired, full length V(D)J receptor sequences in the same cells
- Simultaneously measure additional biological information with Feature Barcode technology
- Link full-length, paired TCR α and β chain sequences with TCR-pMHC antigen specificity
- Simultaneously measure cell surface protein expression
- Easy to install and use pipelines and visualization software
Currently available Feature Barcode kits for Single Cell Immune Profiling HT Feature Barcode Technology
| 10x Solution |
Gene Expression |
Cell Surface Protein & Immune Receptor Mapping |
CRISPR Screening |
CellPlex |
| Single Cell 5' HT v2 (Dual Index) |
✓ |
✓ |
- |
- |
User Guides
Find your Single Cell Immune Profiling HT User Guide
Technical Notes
Chromium Next GEM Single Cell 5' HT v2: Reagents, Workflow & Data Overview
How-to Video
How-to Video Series: Chromium Next GEM Single Cell 5' HT v2 - Dual Index
Demonstrated Protocols
Immune Profiling HT Demonstrated Protocol Compatibility Table
Sequencing
Sequencing Requirements for Immune Profiling HT
SDS
Chromium Next GEM Single Cell 5’ HT Reagent Kits v2 Safety Data Sheets (HT v2 Dual Index Chemistry)
Software
What is HT (High Throughput)?