Targeted Visium Spatial Gene Expression: Pre-designed Panel Performance Metrics

Technical Note, Last Modified on October 27, 2020, Permalink

CG000364_PerformanceofPredesignedPanelsSpatialLibraries_TN_RevA.pdf

The Targeted Gene Expression product is a modular enrichment kit designed to enrich libraries for relevant genes, while decreasing sequencing requirements by up to 90%. Pre-designed panels from 10x Genomics are used in the Targeted Gene Expression workflow to target over 1,000 genes per panel. When used to enrich whole transcriptome Visium Spatial Gene Expression libraries, these panels enable significantly reduced sequencing costs while accurately reflecting gene expression information from the corresponding whole transcriptome analysis (WTA) parent library for targeted genes. This Technical Note describes the performance metrics for pre-designed panels as tested across several human tissue types. This document shows that the use of pre-designed panels with targeted libraries results in even enrichment and accurate representation of on target molecules relevant to the corresponding Visium library.

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