Methanol Fixation, Immunofluorescence Staining & Imaging for Visium Spatial Protocols
Demonstrated Protocol, Last Modified on February 15, 2022, Permalink
CG000312_Demonstrated Protocol Methanol Fixation and IF Staining_RevD.pdf
The Visium Spatial Gene Expression Solution measures the total mRNA in tissue sections and requires a Visium Spatial slide with intact tissue sections as input. Immunostaining tissue sections with fluorescently labeled antibodies enables simultaneous protein detection. This protocol outlines methanol fixation, immunofluorescence staining, and imaging of tissue for use with 10x Genomics Visium Spatial protocols. Fixed and stained tissue sections are inputs for the downstream Visium Spatial Tissue Optimization and Visium Spatial Gene Expression workflows.
FOR USE WITH:
- Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides PN-1000193
- Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns PN-1000184
- Visium Spatial Gene Expression Slide & Reagent Kit, 4 rxns PN-1000187
- Visium Gateway Tissue Optimization Slide & Reagent Kits, 1 slide (PN-1000313, PN-1000314)
- Visium Gateway Gene Expression Slide & Reagent Kit, 2 rxns PN-1000317
- Visium Accessory Kit, PN-1000194
- Dual Index Kit TT Set A, 96 rxns PN-1000215