10x Genomics
Spatial Gene Expression for FFPE

Space Ranger, printed on 12/22/2024

Visium Human Transcriptome Probe Set v2.0

Probe Set Contents

• 18,536 genes targeted by 54,018 probes.
  • 451 (2.4%) of these genes (targeted by 514 probes) are excluded by default due to predicted off-target activity to a different gene. See the probe filter section of the Space Ranger algorithms overview for more details.
  • 18,085 genes (targeted by 53,504 probes) are present in the final filtered output.

The gene_id information is included in the probe set reference CSV file.

Accompanying Probe Set Files

• Visium Human Transcriptome Probe Set v2.0 - Probe Set Reference CSV file
• Visium Human Transcriptome Probe Set v2.0 - BED file
• Visium Human Transcriptome Probe Set v2.0 - Probe Off-target CSV file
• Visium Human Transcriptome Probe Set v2.0 - Probe Set Metadata TSV file

These files are downloadable from the software downloads section.

For details of the file formats see the probe set file descriptions.

Associated Part Numbers

• Visium Human Transcriptome Probe Kit v2, Small PN-1000466