Visium CytAssist Spatial Gene Expression for FFPE Imaging Guidelines

Technical Note, Last Modified on August 15, 2022, Permalink

CG000521_VisiumCytAssistImagingGuidelinesTN_RevA.pdf

The Visium CytAssist Spatial Gene Expression for FFPE assay is designed to analyze mRNA in tissue sections derived from formalin fixed & paraffin embedded (FFPE) tissue samples. This Technical Note is applicable to both Hematoxylin & Eosin (H&E) and immunofluorescence (IF) stained tissue sections on glass slides. Successful gene expression and protein visualization is highly dependent on good imaging practices. This Technical Note provides hardware recommendations, general image acquisition and analysis guidelines, and examples of images that are suitable for downstream analysis with Space Ranger. Individual results may vary depending on the specific imaging system, and/or sample characteristics.