Cell Labeling with dCODE Dextramer® for Single Cell RNA Sequencing Protocols

Demonstrated Protocol, Last Modified on July 12, 2022, Permalink

CG000203_DemonstratedProtocol_CellLabelingwithDextramers_RevC.pdf

Multimeric MHC peptide complexes, such as dCODE™ Dextramer® reagents, bind to T-cell receptors (TCRs) with high affinity, which can enable detection of TCR antigen specificity. This protocol provides guidance for labeling cells with dCODE™ Dextramer® reagents (dCODE™ Dextramer® MHC-Feature Barcode oligonucleotide conjugate) along with TotalSeq-C antibody-oligonucleotide conjugates. This document also provides guidance for enriching dCODE™ Dextramer®+ T cells by Fluorescence Activated Cell Sorting (FACS). These dCODE™ Dextramer® reagents and TotalSeq-C antibody-oligonucleotide conjugate labeled cells can be used for generating Chromium Single Cell libraries as described in the User Guide for Chromium Single Cell Immune Profiling Solution with Feature Barcode technology (CG000186, CG000208, CG000330, and CG000424).

{"Antigen-only analysis is not currently supported by 10x Genomics. For antigen analysis, we recommend staining with dCODE™ Dextramer® reagents in conjunction with a small panel of TotalSeq-C antibodies. This increases the cell calling accuracy of the data. Please contact 10x Genomics support at support@10xgenomics.com for TotalSeq-C antibody recommendations."}

FOR USE WITH:

• Chromium Next GEM Single Cell 5' HT Reagent Kits v2 (Dual Index) with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping User Guide (Document CG000424)
• Chromium Next GEM Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) with Feature Barcoding technology for Cell Surface Protein and Immune Receptor Mapping (CG000330)
• Chromium Next GEM Single Cell V(D)J Reagent Kits User Guide v1.1 with Feature Barcoding technology for Cell Surface Protein (CG000208)
• Chromium Single Cell V(D)J Reagent Kits User Guide with Feature Barcoding technology for Cell Surface Protein (CG000186)