Interpreting Cell Ranger ARC Web Summary Files for Single Cell Multiome ATAC + Gene Expression Assay

Technical Note, ATAC and Gene Expression, Last Modified on November 6, 2020, Permalink

CG000368_TechnicalNote_InterpretingARCWebSummaryFilesforMultiomeATAC+GEX_RevA.pdf

Technical Note - Interpreting Cell Ranger ARC Web Summary Files for Single Cell Multiome ATAC + Gene ExpressionAssay

The web summary file in the output folder of the Cell Ranger ATAC analysis software is the initial point of reference for determining sample performance in the Single Cell ATAC assay. This Technical Note presents an overview of web summary file interpretation, including the expected metrics and characteristic plots for libraries generated using the Single Cell ATAC workflow.

FOR USE WITH:

• Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns PN-1000283, includes:

  • Chromium Next GEM Single Cell Multiome ATAC Kit A, 16 rxns PN-1000280
  • Chromium Next GEM Single Cell Multiome Reagent Kit A, 16 rxns PN-1000232
  • Library Construction Kit, 16 rxns PN-1000190

• Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns PN-1000285, includes:

  • Chromium Next GEM Single Cell Multiome ATAC Kit A, 4 rxns PN-1000281
  • Chromium Next GEM Single Cell Multiome Reagent Kit A, 4 rxns PN-1000236
  • Library Construction Kit B, 4 rxns PN-1000279

• Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234

• Chromium Next GEM Chip J Single Cell Kit, 16 rxns PN-1000230

• Single Index Kit N Set A, 96 rxns PN-1000212

• Dual Index Kit TT Set A, 96 rxns PN-1000215