Sequencing Metrics & Base Composition of Single Cell 3' v3.1 Dual Index Libraries

Technical Note, v3.1 Next GEM Chemistry, Last Modified on March 22, 2022, Permalink

CG000374_TechNote_SeqMetrics_SingleCell3-v3.1DualIndex_RevD.pdf

The Chromium Next GEM Single Cell 3' v3.1 (dual index) workflow produces sequencing-ready Gene Expression libraries alone or in combination with either Cell Surface Protein or CRISPR Screening libraries from the same single cells. This enables simultaneous profiling of cellular features in combination with gene expression profiling. This Technical Note presents a comparison of sequencing metrics for various Single Cell 3' Dual Index library types across Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.

FOR USE WITH:

• Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns PN-1000268
• Chromium Next GEM Single Cell 3ʹ Kit v3.1, 4 rxns PN-1000269
• Chromium Next GEM Single Cell 3' HT Kit v3.1, 48 rxns PN-1000348
• Chromium Next GEM Single Cell 3' HT Kit v3.1, 8 rxns PN-1000370
• 3’ Feature Barcode Kit, 16 rxns PN-1000262
• 3ʹ CellPlex Kit Set A, 48 rxns PN-1000261
• Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120
• Chromium Next GEM Chip G Single Cell Kit, 16 rxns PN-1000127
• Chromium Next GEM Chip M Single Cell Kit, 80 rxns PN-1000349
• Chromium Next GEM Chip M Single Cell Kit, 16 rxns PN-1000371
• Dual Index Kit TT Set A, 96 rxns PN-1000215
• Dual Index Kit NT Set A, 96 rxns PN-1000242
• Dual Index Kit NN Set A, 96 rxns PN-1000243