Best Practices to Minimize Chromium Next GEM Chip Clogs and Wetting Failures

Technical Note, Last Modified on December 20, 2021, Permalink

CG000479_TechNote_MinimizingClogs&WettingFailures_RevB.pdf

Chromium Next GEM technology enables profiling the whole transcriptome of hundreds to tens of thousands of cells per sample. In combination with multiomic approaches such as Feature Barcode technology, CellPlex technology, and Immune profiling, a broad landscape of biological questions can be interrogated. This Technical Note highlights sample preparation best practices to minimize microfluidic chip clogs and wetting failures to enable optimal 10x Genomics Single Cell assay performance.