HMW DNA Extraction from Tissue

High Input Genomic DNA Length Results in Optimal Performance of the Chromium™ Genome Protocols

Demonstrated Protocol, Last Modified on January 18, 2017, Permalink

20160607_SamplePrepDemonstratedProtocol - DNAExtractionfromTissue_RevB.pdf

The Chromium Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight (HMW) genomic DNA (gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Optimal performance has been characterized on input gDNA with a mean length >50 kb.

This Demonstrated Protocol outlines the recommended method for HMW gDNA extraction from ~25 mg tissue. No additional reagents or consumables are required other than those listed in the Chromium Genome User Guides.

This Demonstrated Protocol can produce gDNA with an average size of ~20-80 kb when analyzed on a pulsed-field gel, and typically ~40 kb after the Chromium Genome Protocols. There are many variables that will affect gDNA quality in these sample types, including sample age, transportation methods, additives, freezing method, etc.