Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling

10x Genomics Sample Preparation Products, Fixed RNA Profiling, Last Modified on May 17, 2022, Permalink

CG000478_DemonstratedProtocol_Cell&NucleiFixation_Chromium_FixedRNA_Profiling_RevA.pdf

Chromium Fixed RNA Profiling offers comprehensive scalable solutions to measure gene expression in single cell and nuclei suspensions that are fixed with formaldehyde. This protocol outlines how to perform fixation on single cell and nuclei suspensions for use with Chromium Fixed RNA Profiling workflow. This protocol also provides guidance on storage of fixed cells and post-storage processing.

Prior to fixation, samples can also be labeled using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide. Consult Demonstrated Protocol Cell Surface Protein Labeling for Chromium Fixed RNA Profiling with Feature Barcode technology (CG000529) for guidance. Please note: Cell Surface Protein Labeling for Chromium Fixed RNA Profiling with Feature Barcode technology is only compatible with Singleplexed samples.